期刊
ANNALS OF THE RHEUMATIC DISEASES
卷 77, 期 4, 页码 579-588出版社
BMJ PUBLISHING GROUP
DOI: 10.1136/annrheumdis-2017-212279
关键词
autoimmune diseases; early rheumatoid arthritis; rheumatoid arthritis; T cells
类别
资金
- Arthritis Research UK (ARUK) programme [19788]
- IMI
- Arthritis Research UK [20298]
Objectives Successful early intervention in rheumatoid arthritis (RA) with the aim of resetting immunological tolerance requires a clearer understanding of how specificity, cellular kinetics and spatial behaviour shape the evolution of articular T cell responses. We aimed to define initial seeding of articular CD4(+) T cell responses in early experimental arthritis, evaluating their dynamic behaviour and interactions with dendritic cells (DCs) in the inflamed articular environment. Methods Antigen-induced arthritis was used to model articular inflammation. Flow cytometry and PCR of T cell receptor (TCR) diversity genes allowed phenotypic analysis of infiltrating T cells. The dynamic interactions of T cells with joint residing DCs were visualised using intravital multiphoton microscopy. Results Initial recruitment of antigen-specific T cells into the joint was paralleled by accumulation of CD4(+) T cells with diverse antigen-receptor expression and ability to produce tumour necrosis factor alpha (TNF) and interferon gamma (IFN) on mitogenic restimulation. A proportion of this infiltrate demonstrated slower motility speeds and engaged for longer periods with articular DCs in vivo. Abatacept treatment did not disrupt these interactions but did reduce T cell expression of inducible costimulatory (ICOS) molecule. We also demonstrated that non-specific CD4(+) T cells could be recruited during these early articular events. Conclusions We demonstrate that CD4(+) T cells engage with articular DCs supporting antigen specific T cell reactivation. This cellular dialogue can be targeted therapeutically to reduce local T cell activation.
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