Effects of spatial patterning of co-flowering plant species on pollination quantity and purity

Background and Aims If two plant species share pollinators, it has been proposed that the interaction between them may range from competitive to facilitative, depending on the way in which they intermingle. In particular, the presence of a rewarding plant species may increase the rate of pollinator visitation to a less rewarding species in its vicinity, but the beneficial increase in visitation may be counteracted by a detrimental increase in heterospecific pollen transfer. We assessed this trade-off using bumble-bees foraging over a gradual spatial transition between two plant species in an indoor cage experiment. Methods We used two species' of artificial flowers - one more rewarding than the other - in arrays that varied in the degree of species intermingling. The flowers dispensed and received powdered food dyes serving as pollen analogues. Captive bumble-bees visited to collect sucrose solution. We quantified dye delivery to the adhesive-tape stigmas' in flowers by spectrophotometry. Key Results Across the spatial transition between species, the less attractive species received more dye (more bee visits) when in proximity to the more attractive species than it did when alone, but the larger dye loads were less pure (more heterospecific pollen transfer). The decline in purity cancelled out the gain in acquisition, so conspecific pollen receipt by the less attractive species was neutrally affected. The more attractive species received fewer visits when surrounded by the less attractive species, so the interaction between the two species was amensalism when considering conspecific pollen reception. Conclusions Pollinator-mediated interactions between plant species depend on pollination quantity and purity, both of which can depend on spatial intermingling.