期刊
ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER
卷 216, 期 -, 页码 29-35出版社
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.aanat.2017.10.001
关键词
Periodic acid-Schiff stain; Fascia; Whole-body slice plastination; Cross-sectional anatomy; Collagen; Tendon; Aponeurosis
资金
- Stiftung Gynakologisch-Onkologische Forschung
Since collagen rich fascial tissue is often very delicate and difficult to discern on native tissue slices, we have developed a method for staining full-body slices using the periodic acid-Schiff (PAS) reaction with subsequent plastination. Since the PAS reaction primarily stains carbohydrates, we could exploit the circumstance that different collagen types vary in carbohydrate content. Contrary to fasciae, tissues such as muscle, bone, nerves and blood vessels exhibit significantly less staining or remain unstained. We have validated the whole-body slice staining results in microscopic tissue slides which were stained with standard extracellular matrix stains such as Masson-Goldner trichrome stain and van-Gieson stain. Furthermore, we have performed immunofluorescence imaging to confirm the presence of collagen in the stained tissue. We achieved very good staining and plastination results and were able to clearly identify even very thin fascia in transversal body slices. This technique may prove useful in advancing our knowledge on the complex topography of fascial structures. (C) 2017 Elsevier GmbH. All rights reserved.
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