期刊
BREAST CANCER RESEARCH
卷 18, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s13058-016-0703-7
关键词
Extracellular matrix; Tumor microenvironment; Collagen; Tumor associated neutrophils; Breast cancer; MMTV-PyVT; Stroma; Cytokines
类别
资金
- University of Wisconsin Translational Research Initiatives in Pathology Laboratory
- National Science Foundation (NSF) Graduate Research Fellowship Program (GRFP) awarded [DGE-1256259, R01 CA114462, 1R01 CA142833]
- UW Department of Pathology and Laboratory Medicine and UWCCC
- UWCCC Flow Cytometry Special BD LSR Fortessa Grant [1S100OD018202-01]
- [P30 CA014520]
Background: High mammographic density has been correlated with a 4-fold to 6-fold increased risk of developing breast cancer, and is associated with increased stromal deposition of extracellular matrix proteins, including collagen I. The molecular and cellular mechanisms responsible for high breast tissue density are not completely understood. Methods: We previously described accelerated tumor formation and metastases in a transgenic mouse model of collagen-dense mammary tumors ( type I collagen-alpha 1 (Col1 alpha 1)(tm1Jae) and mouse mammary tumor virus - polyoma virus middle T antigen (MMTV-PyVT)) compared to wild-type mice. Using ELISA cytokine arrays and multi-color flow cytometry analysis, we studied cytokine signals and the non-malignant, immune cells in the collagen-dense tumor microenvironment that may promote accelerated tumor progression and metastasis. Results: Collagen-dense tumors did not show any alteration in immune cell populations at late stages. The cytokine signals in the mammary tumor microenvironment were clearly different between wild-type and collagendense tumors. Cytokines associated with neutrophil signaling, such as granulocyte monocyte-colony stimulated factor (GM-CSF), were increased in collagen-dense tumors. Depleting neutrophils with anti-Ly6G (1A8) significantly reduced the number of tumors, and blocked metastasis in over 80 % of mice with collagen-dense tumors, but did not impact tumor growth or metastasis in wild-type mice. Conclusion: Our study suggests that tumor progression in a collagen-dense microenvironment is mechanistically different, with pro-tumor neutrophils, compared to a non-dense microenvironment.
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