期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 57, 期 32, 页码 10268-10272出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201806941
关键词
black phosphorus; CRISPR/Cas9; gene silencing; genome editing; two-dimensional materials
资金
- National Natural Science Foundation of China [31600595, 51672305]
- International Cooperation Project for Science and Research Plan of Shenzhen [GJHZ20160229195805334]
- Frontier Science Key Programs of Chinese Academy of Sciences [QYZDB-SSW-SLH034]
- Leverhulme Trust [RPG-2015-345]
A biodegradable two-dimensional (2D) delivery platform based on loading black phosphorus nanosheets (BPs) with Cas9 ribonucleoprotein engineered with three nuclear localization signals (NLSs) at C terminus (Cas9N3) is successfully established. The Cas9N3-BPs enter cells effectively via membrane penetration and endocytosis pathways, followed by a BPs biodegradation-associated endosomal escape and cytosolic releases of the loaded Cas9N3 complexes. The Cas9N3-BPs thus provide efficient genome editing and gene silencing invitro and invivo at a relatively low dose as compared with other nanoparticle-based delivery platforms. This biodegradable 2D delivery platform offers a versatile cytosolic delivery approach for CRISPR/Cas9 ribonucleoprotein and other bioactive macromolecules for biomedical applications.
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