期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 57, 期 32, 页码 10137-10141出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201804731
关键词
labeling agents; near-infrared; optical imaging; phospha-rhodamine; photoresistance
资金
- JSPS KAKENHI [17H05839, 16K13097, JP15H05952, JP15H04962, 16H05119, 17H19511, JP16H06280]
- JST CREST [JPMJCR15G2]
- JSPS
Various fluorescence microscopy techniques require bright NIR-emitting fluorophores with high chemical and photostability. Now, the significant performance improvement of phosphorus-substituted rhodamine dyes (PORs) upon substitution at the 9-position with a 2,6-dimethoxyphenyl group is reported. The thus obtained dye PREX 710 was used to stain mitochondria in living cells, which allowed long-term and three-color imaging in the vis-NIR range. Moreover, the high fluorescence longevity of PREX 710 allows tracking a dye-labeled biomolecule by single-molecule microscopy under physiological conditions. Deep imaging of blood vessels in mice brain has also been achieved using the bright NIR-emitting PREX 710-dextran conjugate.
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