期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 57, 期 30, 页码 9346-9350出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201804309
关键词
fluorescence; fluorescent probes; oxidative stress; reactive sulfur species; redox biology
资金
- AMED-CREST
- JST, PRESTO
- MEXT/JSPS KAKENHI [JP16H02606, JP26111012, JP15H05951]
- JSPS Core-to-Core Program
- Hoansha Foundation
The chemical biology of reactive sulfur species, including hydropolysulfides, has been a subject undergoing intense study in recent years, but further understanding of their intact function in living cells has been limited owing to a lack of appropriate analytical tools. In order to overcome this limitation, we developed a new type of fluorescent probe that reversibly and selectively reacts to hydropolysulfides. The probe enables live-cell visualization and quantification of endogenous hydropolysulfides without interference from intrinsic thiol species such as glutathione. Additionally, real-time reversible monitoring of oxidative-stress-induced fluctuation of intrinsic hydropolysulfides has been achieved with a temporal resolution on the order of seconds, a result which has not yet been realized using conventional methods. These results reveal the probe's versatility as a new fluorescence imaging tool to understand the function of intracellular hydropolysulfides.
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