期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 57, 期 30, 页码 9470-9474出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201802890
关键词
atomic force microscopy; DNA nanotechnology; DNA origami; magnesium; stability
资金
- Academy of Finland [286845, 308578]
- Jane and Aatos Erkko Foundation
- Sigrid Juselius Foundation
- Deutsche Forschungsgemeinschaft [KE 1944/2-1]
- Academy of Finland's Centres of Excellence Programme
DNA origami structures have great potential as functional platforms in various biomedical applications. Many applications, however, are incompatible with the high Mg2+ concentrations commonly believed to be a prerequisite for maintaining DNA origami integrity. Herein, we investigate DNA origami stability in low-Mg2+ buffers. DNA origami stability is found to crucially depend on the availability of residual Mg2+ ions for screening electrostatic repulsion. The presence of EDTA and phosphate ions may thus facilitate DNA origami denaturation by displacing Mg2+ ions from the DNA backbone and reducing the strength of the Mg2+-DNA interaction, respectively. Most remarkably, these buffer dependencies are affected by DNA origami superstructure. However, by rationally selecting buffer components and considering superstructure-dependent effects, the structural integrity of a given DNA origami nanostructure can be maintained in conventional buffers even at Mg2+ concentrations in the low-micromolar range.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据