期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 57, 期 7, 页码 1817-1820出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201711710
关键词
chemoproteomics; labeling; O-GlcNAc; S-glycosylation; unnatural monosaccharides
资金
- National Key Research and Development Projects [2016YFA0501500]
- National Natural Science Foundation of China [21425204, 21521003, 21672013, 21472008, 81490740]
- 1000 Talents Plan Young Investigator Award
The unexpected, non-enzymatic S-glycosylation of cysteine residues in various proteins by per-O-acetylated monosaccharides is described. This artificial S-glycosylation greatly compromises the specificity and validity of metabolic glycan labeling in living cells by per-O-acetylated azido and alkynyl sugars, which has been overlooked in the field for decades. It is demonstrated that the use of unacetylated unnatural sugars can avoid the artifact formation and a corrected list of O-GlcNAcylated proteins and O-GlcNAc sites in HeLa cells has been assembled by using N-azidoacetylgalactosamine (GalNAz).
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据