期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 57, 期 19, 页码 5418-5422出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201801498
关键词
DNA nanotechnology; molecular channels; molecular dynamics; SAXS; shape reconfiguration
资金
- National Key R&D Program of China [2016YFA0201200, 2016YFA0400900]
- Key Research Program of Frontier Sciences, CAS [QYZDJ-SSW-SLH031]
- National Science Foundation of China [21390414, 21329501, 31371015, 21603262, 21373260, 31070640]
- Natural Science Foundation of Shanghai [17ZR1442200]
- NIH [R01GM114056]
Reconfiguration of membrane protein channels for gated transport is highly regulated under physiological conditions. However, a mechanistic understanding of such channels remains challenging owing to the difficulty in probing subtle gating-associated structural changes. Herein, we show that charge neutralization can drive the shape reconfiguration of a biomimetic 6-helix bundle DNA nanotube (6HB). Specifically, 6HB adopts a compact state when its charge is neutralized by Mg2+; whereas Na+ switches it to the expanded state, as revealed by MD simulations, small-angle X-ray scattering (SAXS), and FRET characterization. Furthermore, partial neutralization of the DNA backbone charges by chemical modification renders 6HB compact and insensitive to ions, suggesting an interplay between electrostatic and hydrophobic forces in the channels. This system provides a platform for understanding the structure-function relationship of biological channels and designing rules for the shape control of DNA nanostructures in biomedical applications.
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