期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 57, 期 20, 页码 5750-5753出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201801653
关键词
aggregation-induced emission; metal-ion sensor; protein detection; silver staining; tetrazolate-silver assembly
资金
- Innovation and Technology Commission [ITC-CNERC14SC01]
- University Grants Committee of Hong Kong [AoE/P-03/08]
- Research Grants Council of Hong Kong [16301614, 16305015, N_HKUST604/14, A-HKUST605/16]
- National Science Foundation of China [21788102]
- Swedish Research Council [2017-06344]
Silver staining, which exploits the special bioaffinity and the chromogenic reduction of silver ions, is an indispensable visualization method in biology. It is a most popular method for in-gel protein detection. However, it is limited by run-to-run variability, background staining, inability for protein quantification, and limited compatibility with mass spectroscopic (MS) analysis; limitations that are largely attributed to the tricky chromogenic visualization. Herein, we reported a novel water-soluble fluorogenic Ag+ probe, the sensing mechanism of which is based on an aggregation-induced emission (AIE) process driven by tetrazolate-Ag+ interactions. The fluorogenic sensing can substitute the chromogenic reaction, leading to a new fluorescence silver staining method. This new staining method offers sensitive detection of total proteins in polyacrylamide gels with a broad linear dynamic range and robust operations that rival the silver nitrate stain and the best fluorescent stains.
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