期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 57, 期 8, 页码 2165-2169出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201712284
关键词
DNA; i-motifs; in-cell NMR spectroscopy; structural biology
资金
- Czech Science Foundation [16-10504S]
- European Regional Development Fund [CZ.02.1.01/0.0/0.0/15_003/0000477, CZ.02.1.01/0.0/0.0/15_003/0000441]
- European Molecular Biology Organization [IG2535]
- Horizon 2020 Programme of the EU (iNEXT) [653706]
- MEYS CR (CEITEC 2020) [LQ1601]
- Czech-BioImaging [LM2015062]
- [CIISB-LM2015043]
- [EATRIS-CZ LM2015064]
C-rich DNA has the capacity to form a tetra-stranded structure known as an i-motif. The i-motifs within genomic DNA have been proposed to contribute to the regulation of DNA transcription. However, direct experimental evidence for the existence of these structures invivo has been missing. Whether i-motif structures form in complex environment of living cells is not currently known. Herein, using state-of-the-art in-cell NMR spectroscopy, we evaluate the stabilities of i-motif structures in the complex cellular environment. We show that i-motifs formed from naturally occurring C-rich sequences in the human genome are stable and persist in the nuclei of living human cells. Our data show that i-motif stabilities invivo are generally distinct from those invitro. Our results are the first to interlink the stability of DNA i-motifs invitro with their stability invivo and provide essential information for the design and development of i-motif-based DNA biosensors for intracellular applications.
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