期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 57, 期 25, 页码 7503-7507出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201801815
关键词
Click Chemistry; enzyme assembly; lab-on-a-chip immunoassays; tunable detection range
资金
- National Science Foundation of China [81671784, 21505027, 81361140345, 21535001, 81730051, 21761142006]
- Ministry of Science and Technology of China [2013YQ190467]
- Beijing Nova Program [Z181100006218017]
- Chinese Academy of Sciences [2018047, XDA09030305, 121D11KYSB20170026]
Multiplexed analysis of molecules with different concentrations requires assays with a tunable detection range. A strategy is outlined that uses click chemistry to assemble horseradish peroxidase in a controlled fashion to generate enzyme assemblies as probes for multiplexed bioassays. This controllable assembly of enzymes on detection antibodies allows for lab-on-a-chip immunoassays with a tunable detection range from pgmL(-1) to gmL(-1). Simultaneous, multiplexed bioassays of clinically relevant inflammatory biomarkers in serum are demonstrated in one lab-on-a-chip format, with a limit of detection of 0.47pgmL(-1) for interleukin-6, 2.6 pgmL(-1) for procalcitonin, and 40ngmL(-1) for C-reactive protein. This controlled assembly technique provides a multiplexed platform for simultaneous and quantitative analyses of both low-abundance and high-abundance biomarkers with a broad detection range, which holds great promise as a point-of-care platform for biomedical diagnostics.
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