期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 57, 期 24, 页码 7131-7135出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201802701
关键词
biosensors; cfDNA; DNA nanostructure; framework nucleic acids; valence-engineering
资金
- NSFC [31470960, 21390414, 21722502, 21505045, 21705048, 21475152]
- Shanghai Municipal Education Commission-Gaofeng Clinical Medicine Grant Support [20171913]
- Ministry of Science and Technology of China [2016YFA0201200, 2016YFA0400900]
- Chinese Academy of Sciences [QYZDJ-SSW-SLH031]
Weak ligand-receptor recognition events are often amplified by recruiting multiple regulatory biomolecules to the action site in biological systems. However, signal amplification in invitro biomimetic systems generally lack the spatiotemporal regulation invivo. Herein we report a framework nucleic acid (FNA)-programmed strategy to develop valence-controlled signal amplifiers with high modularity for ultrasensitive biosensing. We demonstrated that the FNA-programmed signal amplifiers could recruit nucleic acids, proteins, and inorganic nanoparticles in a stoichiometric manner. The valence-controlled signal amplifier enhanced the quantification ability of electrochemical biosensors, and enabled ultrasensitive detection of tumor-relevant circulating free DNA (cfDNA) with sensitivity enhancement of 3-5 orders of magnitude and improved dynamic range.
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