期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 57, 期 29, 页码 9003-9007出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201804373
关键词
chemoenzymatic synthesis; dystroglycan; glycosylation; glycosyltransferases; O-mannose glycans
资金
- National Natural Science Foundation of China [21372145, 21672128, 21702123]
- State Key Laboratory of Microbial Technology [M2016-06]
- Department of Science and Technology of Shandong Province [2016GGH4502, 2016GSF121002]
- NIH [R01GM032373]
O-Mannose glycans account up to 30% of total O-glycans in the brain. Previous synthesis and functional studies have only focused on the core M3 O-mannose glycans of -dystroglycan, which are a causative factor for various muscular diseases. In this study, a highly efficient chemoenzymatic strategy was developed that enabled the first collective synthesis of 63 core M1 and core M2 O-mannose glycans. This chemoenzymatic strategy features the gram-scale chemical synthesis of five judiciously designed core structures, and the diversity-oriented modification of the core structures with three enzyme modules to provide 58 complex O-mannose glycans in a linear sequence that does not exceed four steps. The binding profiles of synthetic O-mannose glycans with a panel of lectins, antibodies, and brain proteins were also explored by using a printed O-mannose glycan array.
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