期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 57, 期 20, 页码 5645-5649出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201800032
关键词
activity-based probes; chemical proteomics; dehydroalanine; protein modifications; ubiquitin
资金
- Israel Science Foundation
- Taub Foundation
We report a general and novel semisynthetic strategy for the preparation of ubiquitinated protein-activity-based probes on the basis of sequential dehydroalanine formation on expressed proteins. We applied this approach to construct a physiologically and therapeutically relevant ubiquitinated alpha-globin probe, which was used for the enrichment and proteomic identification of alpha-globin-modulating deubiquitinases. We found USP15 as a potential deubiquitinase for the modulation of alpha-globin, an excess of which aggravates beta-thalassemia symptoms. This development opens new opportunities for activity-based-probe design to shed light on the important aspects underlying ubiquitination and deubiquitination in health and disease.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据