期刊
FEMS YEAST RESEARCH
卷 16, 期 4, 页码 -出版社
OXFORD UNIV PRESS
DOI: 10.1093/femsyr/fow044
关键词
novel yeast species; Spathaspora; D-xylose fermentation; genome sequencing; enzymes
资金
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq-Brazil) [560715/2010-2, 141563/2011-7, 552877/2007-7, 457499/2014-1]
- Fundacao do Amparo a Pesquisa do Estado de Minas Gerais
- Financiadora de Estudos e Projetos (FINEP) [2084/07]
- Natural Science and Engineering Research Council of Canada
- US National Science Foundation [DEB-1442148]
- DOE Great Lakes Bioenergy Research Center (US DOE Office of Science) [BER DE-FC02-07ER64494]
- USDA National Institute of Food and Agriculture [1003258]
- Pew Charitable Trusts
- Alexander von Humboldt Foundation
- Direct For Biological Sciences
- Division Of Environmental Biology [1442148] Funding Source: National Science Foundation
Three novel D-xylose-fermenting yeast species of Spathaspora clade were recovered from rotting wood in regions of the Atlantic Rainforest ecosystem in Brazil. Differentiation of new species was based on analyses of the gene encoding the D1/D2 sequences of large subunit of rRNA and on 642 conserved, single-copy, orthologous genes from genome sequence assemblies from the newly described species and 15 closely-related Debaryomycetaceae/Metschnikowiaceae species. Spathaspora girioi sp. nov. produced unconjugated asci with a single elongated ascospore with curved ends; ascospore formation was not observed for the other two species. The three novel species ferment D-xylose with different efficiencies. Spathaspora hagerdaliae sp. nov. and Sp. girioi sp. nov. showed xylose reductase (XR) activity strictly dependent on NADPH, whereas Sp. gorwiae sp. nov. had XR activity that used both NADH and NADPH as co-factors. The genes that encode enzymes involved in D-xylose metabolism (XR, xylitol dehydrogenase and xylulokinase) were also identified for these novel species. The type strains are Sp. girioi sp. nov. UFMG-CM-Y302(T) (= CBS 13476), Sp. hagerdaliae f. a., sp. nov. UFMG-CM-Y303(T) (= CBS 13475) and Sp. gorwiae f. a., sp. nov. UFMG-CM-Y312(T) (= CBS 13472).
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