Comparison of Tris egg yolk-based, Triladyl® and Optixell® extender on post-thaw quality, Kinematics and in vivo fertility of Nili Ravi Buffalo (Bubalus bubalis) bull spermatozoa

Our objectives were to ascertain the comparison of Tris egg yolk-based, Triladyl (R) and Optixell (R) extender on postthaw quality, CASA parameters and in vivo fertility of Nili Ravi buffalo (Bubalus bubalis) bull spermatozoa. Semen samples (n = 35) from five bulls were diluted in Tris egg yolk-based, Triladyl (R), Optixell (R) extender and frozen in 0.50 ml French straws. Postthaw sperm CASA motility (%) was higher (p < 0.05) in Optixell extender as compared to Triladyl and Tris egg yolk-based extender. Although sperm progressive motility (%), morphology (%), average path velocity (mu m/s), straight line velocity (mu m/s), curvilinear velocity (mu m/s), amplitude of lateral head displacement (pm), beat cross-frequency (Hz), straightness (%), length of curvilinear path (mu m), length of average path (mu m), intact plasma and acrosome membrane (%), and DNA integrity (%) were higher (p < 0.05) in spermatozoa cryopreserved in Optixell (R) extender as compared to Tris egg yolk-based and Triladyl (R) extender. The fertility rates (68.18%, 45.45%, 55.4%) were higher (p < 0.05) in buffaloes inseminated with semen doses frozen in Optixell extender than the Tris egg yolk-based and Triladyl (R) extender respectively. It is concluded that Optixell (R) extender improves postthaw semen quality and fertility in Nili Ravi buffaloes.