期刊
ANALYTICAL CHEMISTRY
卷 90, 期 7, 页码 4303-4309出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.8b00048
关键词
-
资金
- Shanghai Pujiang Program [16PJ1403200]
- Shanghai Science and Technology Committee [16DZ2260601]
- National Natural Science Foundation of China [11674210]
High-throughput measurements can be achieved using droplet-based assays. In this study, we exploited the principles of wetting behavior and capillarity to guide liquids sliding along a solid surface with hybrid wettability. Oil-covered droplet arrays with uniformly sized and regularly shaped picoliter droplets were successfully generated on hydrophilic-in-hydrophobic patterned substrates. More than ten thousand 31-pL droplets were generated in 5 s without any sophisticated instruments. Covering the droplet arrays with oil during generation not only isolated the droplets from each other but also effectively prevented droplet evaporation. The oil-covered droplet arrays could be stored for more than 2 days with less than 35% volume loss. Single microspheres, microbial cells, or mammalian cells were successfully captured in the droplets. We demonstrate that Escherichia coli could be encapsulated at a certain number (1-4) and cultured for 3 days in droplets. Cell population and morphology were dynamically tracked within individual droplets. Our droplet array generation method enables high throughput processing and is facile, efficient, and low-cost; in addition, the prepared droplet arrays have enormous potential for applications in chemical and biological assays.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据