期刊
ANALYTICAL CHEMISTRY
卷 90, 期 4, 页码 2891-2895出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.7b05112
关键词
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资金
- Japan Society for the Promotion of Science (JSPS) [15K20856, 15H05422]
- Takeda Science Foundation
- Nakatani Foundation
- Kanazawa University CHOZEN Project
- Development of Systems and Technology for Advanced Measure-ment and Analysis from AMED (The Japan Agency for Medical Research and Development)-SENTAN
- PREST from the Japan Science and Technology Agency (JST)
- BBSRC [BB/M022080/1, BB/L005816/1, BB/D020875/1] Funding Source: UKRI
- EPSRC [EP/P011985/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/M022080/1] Funding Source: researchfish
- Grants-in-Aid for Scientific Research [15K20856] Funding Source: KAKEN
Primary cilia are hair-like sensory organelles whose dimensions and location vary with cell type and culture condition. Herein, we employed scanning ion conductance microscopy (SICM) to visualize the topography of primary cilia from different cell types. By combining SICM with fluorescence imaging, we successfully distinguished between surface cilia that project outward from the cell surface and subsurface cilia that are trapped below it. The nanoscale structure of the ciliary pocket, which cannot be easily identified using a confocal fluorescence microscope, was observed in SICM images. Furthermore, we developed a topographic reconstruction method using current-distance profiles to evaluate the relationship between set point and topographic image and found that a low set point is important for detecting the true topography of a primary cilium using hopping mode SICM.
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