期刊
ANALYTICAL CHEMISTRY
卷 90, 期 3, 页码 1566-1571出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.7b04549
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资金
- Academia Sinica and the Ministry of Science and Technology, Taiwan [104-2811-M-001-149]
- Academia Sinica and the Ministry of Science and Technology, Taiwan [104-2811-M-001-149]
Containing an ensemble of nitrogen-vacancy centers in crystal matrices, fluorescent nanodiamonds (FNDs) are a new type of photostable markers that have found wide applications in light microscopy. The nanomaterial also has a dense carbon core, making it visible to electron microscopy. Here, we show that FNDs encapsulated in biotinylated lipids (bLs) are useful for subdiffraction imaging of antigens on cell surface with correlative light-electron microscopy (CLEM). The lipid encapsulation enables not only good dispersion of the particles in biological buffers but also high specific labeling of live cells. By employing the bL-encapsulated FNDs to target CD44 on HeLa cell surface through biotin-mediated immunostaining, we obtained the spatial distribution of these antigens by CLEM with a localization accuracy of similar to 50 nm in routine operations. A comparative study with dual-color imaging, in which CD44 was labeled with FND and MICA/MICB was labeled with Alexa Fluor 488, demonstrated the superior performance of FNDs as fluorescent fiducial markers for CLEM of cell surface antigens.
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