期刊
ANALYTICAL CHEMISTRY
卷 90, 期 8, 页码 4982-4986出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.8b00502
关键词
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资金
- NIH Biotechnology training grant [5T32GM008359-26]
- American Association of Pharmaceutical Scientists Foundation
Protein-particle conjugates and mixtures have been investigated extensively for their diverse applications in biotechnology. However, general methods to measure protein concentration of protein-particle solutions are lacking. Typically, proteins in turbid solutions require separation or staining with another chromophore to quantitate their concentration. Here we demonstrate a label-free, direct approach to measure protein concentrations in turbid solutions using a UV-vis integrating cavity absorbance spectrometer. Three systems are used to test the ability to measure accurate protein concentrations: proteins adsorbed to Alhydrogel, proteins in solution with gold nanoparticles, and proteins encapsulated within polymeric microspheres. Protein concentrations in each of the three protein-particle systems were successfully quantified using a calibration curve created from the absorbance at 280 nm.
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