期刊
ANALYTICAL CHEMISTRY
卷 90, 期 5, 页码 3556-3562出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.7b05454
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资金
- National Natural Science Foundation of China [21775166, 21505161]
- Fundamental Research Funds for the Central Universities [2632017ZD10]
- Natural Science Foundation of Jiangsu Province [BK20150701]
- Six Talent Peaks Project in Jiangsu Province
The development of well-designed nanoprobes for specific imaging of multiple biomarkers in renal cells will afford beneficial information related to the transmutation process of drug-induced kidney injury (DIKI). However, the most reported nanoprobes for DIKI detection were dependent on single-signal output and lack of kidney targeting. In this work, we reported a renal cell targeting and dual-signal nanoprobe by encapsulating Brite 670 and Dabcyl-KFFFDEVDK-FAM into a low molecular weight chitosan nanoparticle. Confocal fluorescence imaging results demonstrated that the nanoprobe could visualize the upregulation of hydroxyl radical in early stage and activation of caspase-3 in late stage of DIKI at both the renal cell and tissue level. In a mouse DIKI model, the positive time of 8 h using nanoprobe imaging was superior to that of 72 h for serum creatinine or blood urea nitrogen, 16 h for cystatin-C, and 24 h for kidney injury molecule-1 with conventional methods. These results demonstrated that the nanoprobe may be a promising tool for effective early prediction and discriminative imaging of DIKI.
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