期刊
ANALYTICAL CHEMISTRY
卷 90, 期 11, 页码 7018-7024出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.8b01406
关键词
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资金
- National Natural Science Foundation of China [21672131, 21775096, 21705102]
- Talents Support Program of Shanxi Province [2014401]
- Shanxi Province Foundation for Returnees [2017026]
- Scientific Instrument Center of Shanxi University
Herein we utilize the similar though divergent nucleophilic properties of cysteine, homocysteine, and glutathione to achieve the selective detection of cysteine under mildly acidic conditions. This enables the specific in situ detection of lysosomal cysteine. Employing time-dependent fluorescent imaging of probe-labeled AS49 cells, we demonstrate that dexamethasone-induced apoptosis is not dependent on lysosomal cysteine. This methodology can thus produce useful information about pathogenesis associated with cysteine and lysosomes.
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