期刊
ANALYTICAL CHEMISTRY
卷 90, 期 11, 页码 6742-6748出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.8b00581
关键词
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资金
- National Natural Science Foundation of China [21725503, 21575038, 21505120, 21475038]
- Foundation for Innovative Research Groups of NSFC [21521063]
- Young Top-notch Talent for Ten Thousand Talent Program
- Fundamental Research Funds for the Central Universities
- Hunan Provincial Innovation Foundation for Postgraduate [CX2016B117]
As one of the emerging inorganic graphene analogues, two-dimensional titanium carbide (Ti3C2) nanosheets have attracted extensive attention in recent years because of their remarkable structural and electronic properties. Herein, a sensitive and selective nanoprobe to fluorescently probe phospholipase D activity was developed on the basis of an ultrathin Ti3C2 nanosheets-mediated fluorescence quenching effect. Ultrathin Ti3C2 nanosheets with nm in thickness were synthesized from bulk Ti3AlC2 powder by a two-step exfoliation procedure and further modified by a natural phospholipid that is doped with rhodamine B-labeled phospholipid (RhB-PL-Ti3C2). The close proximity between RhB and Ti3C2 leads to efficient fluorescence quenching (>95%) of RhB by energy transfer. Phospholipase D-catalyzed lipolysis of the phosphodiester bond in RhB-PL results in RhB moving away from the surface of Ti3C2 nanosheets and subsequent fluorescence recovery of RhB, providing a fluorescent switch-on assay for the phospholipase D activity. The proposed nanoprobe was successfully applied to quantitatively determine phospholipase D activity with a low limit of detection (0.10 U L-1) and to measure its inhibition. Moreover, in situ monitoring and imaging the activity of phospholipase D in living cells were achieved using this biocompatible nanoprobe. These results reveal that Ti3C2 nanosheets-based probes exhibit great potential in fluorometric assay and clinical diagnostic applications.
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