期刊
ANALYTICAL CHEMISTRY
卷 90, 期 12, 页码 7617-7625出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.8b01369
关键词
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资金
- Science and Technology Commission of Shanghai Municipality (STCSM) [16520710800]
- Fundamental Research Funds for the Central Universities [222201817022]
- Natural Science Foundation of Shanghai [16ZR1407600]
- Shanghai Sailing Program [16YF1402400]
- Australian Research Council, Australian National Fabrication Facility at The University of Queensland
Selective enrichment and sensitive detection of phosphopeptides are of great significance in many bioapplications. In this work, dendritic mesoporous silica nanoparticles modified with polydopamine and chelated Ti4+ (denoted DMSNs@PDA-Ti4+) were developed to improve the enrichment selectivity of phosphopeptides. The unique central-radial pore structures endowed DMSNs@PDA-Ti4+ with a high surface area (362 m(2) g(-1)), a large pore volume (1.37 cm(3) g(-1)), and a high amount of chelated Ti4+ (75 mu g mg(-1)). Compared with conventional mesoporous silica-based materials with the same functionalization (denoted mSiO(2)@PDA-Ti4+) and commercial TiO2, DMSNs@PDA-Ti4+ showed better selectivity and a lower detection limit (0.2 fmol/mu L). Moreover, 2422 unique phosphopeptides were identified from HeLa cell extracts with a high specificity (>95%) enabled by DMSNs@PDA-Ti4+, better than those in previous reports.
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