期刊
ANALYTICAL BIOCHEMISTRY
卷 542, 期 -, 页码 58-62出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2017.11.010
关键词
Paper-based ELISA; Escherichia coli O157:H7; On-site detection; Foodbome pathogens
资金
- Chinese National Natural Science Foundation [81473018, 81602894, 81602895]
- Development Foundation of Science and Technology in Jilin Province of China [20170204003SF]
Escherichia coil O157:H7 (E. coil O157:H7) has become one of the most dangerous foodborne pathogenic bacteria around the world. Currently, because of the tedious, high-cost and stringent laboratory conditions required, the conventional E. coli O157: H7 detection methods, such as culture-based methods and polymerase chain reaction (PCR), have much limitation. Thus, we developed a novel paper-based enzyme-linked immunosorbent assay (p-ELISA) with shorter operation duration, lower cost, relatively higher sensitivity and wider application. This method required less than 3 h and 5 mu L of sample to complete the detection. The limit of detection (LOD) for E. coil O157:H7 reached 1 x 10(4) CFU/mL with high specificity. To be more suitable for on-site testing, the readout could be rapidly obtained without any expensive instruments. In this study, we chose E. coli O157:H7 as the representative, and our method could provide a platform for determination of other pathogenic bacteria.
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