4.7 Article

One- vs two-phase extraction: re-evaluation of sample preparation procedures for untargeted lipidomics in plasma samples

期刊

ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 410, 期 23, 页码 5859-5870

出版社

SPRINGER HEIDELBERG
DOI: 10.1007/s00216-018-1200-x

关键词

Lipids; Untargeted lipidomics; Extraction; LC-MS; Multivariate analysis

资金

  1. Science, Technology and Innovation Department-COLCIENCIAS from Colombia [6171-71294025]
  2. University Medical Center Groningen

向作者/读者索取更多资源

Lipidomics is a rapidly developing field in modern biomedical research. While LC-MS systems are able to detect most of the known lipid classes in a biological matrix, there is no single technique able to extract all of them simultaneously. In comparison with two-phase extractions, one-phase extraction systems are of particular interest, since they decrease the complexity of the experimental procedure. By using an untargeted lipidomics approach, we explored the differences/similarities between the most commonly used two-phase extraction systems (Folch, Bligh and Dyer, and MTBE) and one of the more recently introduced one-phase extraction systems for lipid analysis based on the MMC solvent mixture (MeOH/MTBE/CHCl3). The four extraction methods were evaluated and thoroughly compared against a pooled extract that qualitatively and quantitatively represents the average of the combined extractions. Our results show that the lipid profile obtained with the MMC system displayed the highest similarity to the pooled extract, indicating that it was most representative of the lipidome in the original sample. Furthermore, it showed better extraction efficiencies for moderate and highly apolar lipid species in comparison with the Folch, Bligh and Dyer, and MTBE extraction systems. Finally, the technical simplicity of theMMC procedure makes this solvent system highly suitable for automated, untargeted lipidomics analysis.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据