期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 410, 期 16, 页码 3779-3788出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-018-1047-1
关键词
Antiepileptic drugs; Polydopamine; Magnetic multi-walled carbon nanotube; Biological matrices
资金
- National Natural Science Foundation of China [21675008]
- Beijing Natural Science Foundation [2132048]
In this study, multi-walled carbon nanotubes were coated on the surface of magnetic nanoparticles modified by polydopamine. The synthesized composite was characterized and applied to magnetic-mu-dispersive solid-phase extraction of oxcarbazepine (OXC), phenytoin (PHT), and carbamazepine (CBZ) from human plasma, urine, and cerebrospinal fluid samples prior to analysis by a high-performance liquid chromatography-photodiode array detector. The extraction parameters were investigated and the optimum condition was obtained when the variables were set to the following: sorbent type, Fe3O4@polyDA-MWCNTs (length < 2 mu m); sample pH, 6; amount of sorbent, 15 mg; sorption time, 1.5 min at room temperature; type and volume of the eluent, 2.5 mL methanol; and salt content, none added. Under the optimized conditions, the calibration curves are linear in the concentration range 2-2000 ng/mL, the limits of detection are in the range 0.4-3.1 ng/mL, and the relative standard deviations and relative recoveries of plasma (spiked at 200 ng/mL) and CSF (spiked at 50 ng/mL) are in the ranges 1.4-8.2% and 92.8-96.5%, respectively. The applicability of the method was successfully confirmed by extraction and determination of OXC, PHT, and CBZ in biological matrices.
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