期刊
ANALYTICA CHIMICA ACTA
卷 1026, 期 -, 页码 140-146出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2018.04.015
关键词
Electrochemical biosensor; alpha-glucosidase; Inhibitor screening; Split aptamer
资金
- National Natural Science Foundation of China [21235003]
- Science Foundation of Jiangsu Province, China [BM2015023]
A reusable biosensor has been fabricated in this work for the assay of a alpha glucosidase activity and the inhibitor screening. In this design, the aptamer of ATP is split as split aptamer 1 (Apt 1) and split aptamer 2 (Apt 2), and Apt 2 can link gold nanoparticles (AuNPs) modified with Apt 1 and 4-aminophenyl-alpha-D-glucopyranoside (pAPG). Consequently, the functional AuNPs can be immobilized onto the surface of gold electrode, allowing for salt-induced regeneration. In the presence of alpha-glucosidase, the glycosyl of pAPG is cut off, and the electroactive phenolic hydroxyls appear to give a strong current signal. Furthermore, the biosensor can be recovered very easily by incubating it in water to dissociate the AuNPs modified with Apt 1 and pAPG. So, a new biosensor for alpha-glucosidase activity detection and inhibitor screening is developed based on enzyme-activated signal generation and recovery. The biosensor may also exhibit good sensitivity for alpha-glucosidase determination with the detection limit 0.005 U/mL and can be reused by water-washing regeneration with good repeatability. Meanwhile this biosensor can also be utilized for inhibitor screening, which may have potential for clinical applications. (C) 2018 Elsevier B.V. All rights reserved.
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