期刊
ANALYTICA CHIMICA ACTA
卷 1004, 期 -, 页码 51-57出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2017.11.079
关键词
Native protein; Nonpolar solvent; Solid substrate; Electrospray ionization; Mass spectrometry
资金
- Natural Science Foundation of China [81373369, 21405127]
- Hong Kong Research Grants Council (CRF) [C5031-14E]
- Hong Kong Research Grants Council (GRF) [5029/13P]
- Science and Technology Planning Project of Guangdong Province [2014A020221106]
Detection of native proteins, particularly directly from raw biological samples, has been a challenging task for mass spectrometry. In this study, we demonstrated that solid-substrate electrospray ionization mass spectrometry with nonpolar solvents such as n-hexane could allow detection of native proteins and protein complexes directly from raw biological samples. Mechanistic study revealed that the process involved rapid vaporization of the nonpolar solvent, temperature reduction of substrate surface, condensation of water from the ambient air, and spray ionization of the condensed water with analytes under the electric field. The fine spray with water at low temperature allowed the technique to detect native proteins, even directly from viscous samples (e.g., egg white) and solid samples (e.g., bone marrow). This study sheds new insight into the sampling and ionization process of mass spectrometry and provides a technique of great potential for characterization of proteins. (c) 2017 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据