期刊
ANALYTICA CHIMICA ACTA
卷 1009, 期 -, 页码 81-88出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2018.01.016
关键词
Point-of-care; Paper-based biosensor; Nucleic acid testing; Electrospinning; Polycaprolactone; Nitrocellulose
资金
- University of Malaya PPP grant [PG037-2015B]
- FRGS from the Ministry of Higher Education, Malaysia [FP054-2015A]
- National Instrumentation Program, People's Republic of China [2013YQ190467]
Point-of-care biosensors are important tools developed to aid medical diagnosis and testing, food safety and environmental monitoring. Paper-based biosensors, especially nucleic acid-based lateral flow assays (LFA), are affordable, simple to produce and easy to use in remote settings. However, the sensitivity of such assays to infectious diseases has always been a restrictive challenge. Here, we have successfully electrospun polycaprolactone (PCL) on nitrocellulose (NC) membrane to form a hydrophobic coating to reduce the flow rate and increase the interaction rate between the targets and gold nanoparticles-detecting probes conjugates, resulting in the binding of more complexes to the capture probes. With this approach, the sensitivity of the PCL electrospin-coated test strip has been increased by approximately ten-fold as compared to the unmodified test strip. As a proof of concept, this approach holds great potential for sensitive detection of targets at point-of-care testing. (c) 2018 Elsevier B.V. All rights reserved.
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