期刊
ANALYTICA CHIMICA ACTA
卷 1023, 期 -, 页码 29-34出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2018.03.060
关键词
Bioconjugation; Electrochemiluminescence; Immunoassay; Iridium complex; Histidine
资金
- Shenzhen Peacock Plan [KQTD2014063010757390]
- Guangdong Innovative and Entrepreneurial Research Team Program [2014ZT05S003]
Histidine bound bis-cyclometalated iridium (III) complexes were found to be luminescent in previous studies. This work demonstrates that the luminophores could be excited electrochemically and the electrogenerated chemiluminescence was observed in both homogeneous solutions and on the surface of magnetic beads. The findings laid a foundation for novel ECL immunoassay methodology featuring site-specific labeling of antibodies and on-site formation of signal-generating units. Taking use of the findings and the unique advantages of the site-specific labeling approach, a sandwich immunoassay for a clinically important biomarker was conceptually proved. The limit of detection of <1 ng/mL procalcitonin in human plasma could be achieved from the unoptimized assay. (C) 2018 Elsevier B.V. All rights reserved.
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