期刊
ANALYTICA CHIMICA ACTA
卷 1018, 期 -, 页码 78-85出版社
ELSEVIER
DOI: 10.1016/j.aca.2018.02.068
关键词
Paper-based test; Nucleic acid extraction; Loop-mediated isothermal amplification; Rotavirus A
资金
- National Natural Science Foundation of China [21505024, 21427806, 21175029, 21335002]
- Introduce Talents of Fudan University Research Funding [JIH1615032]
- Shanghai Leading Academic Discipline Project [B109]
The use of paper-based methods for clinical diagnostics is a rapidly expanding research topic attracting a great deal of interest. Some groups have attempted to realize an integrated nucleic acid test on a single microfluidic paper chip, including extraction, amplification, and readout functions. However, these studies were not able to overcome complex modification and fabrication requirements, long turn-around times, or the need for sophisticated equipment like pumps, thermal cyclers, or centrifuges. Here, we report an extremely simple paper-based test for the point-of-care diagnosis of rotavirus A, one of the most common pathogens that causes pediatric gastroenteritis. This paper-based test could perform nucleic acid extraction within 5 min, then took 25 min to amplify the target sequence, and the result was visible to the naked eye immediately afterward or quantitative by the UV-Vis absorbance. This low-cost method does not require extra equipment and is easy to use either in a lab or at the point-of-care. The detection limit for rotavirus A was found to be 1 x 10(3) copies/mL. In addition, 100% sensitivity and specificity were achieved when testing 48 clinical stool samples. In conclusion, the present paper-based test fulfills the main requirements for a point-of-care diagnostic tool, and has the potential to be applied to disease prevention, control, and precision diagnosis. (c) 2018 Elsevier B.V. All rights reserved.
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