期刊
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
卷 315, 期 1, 页码 L59-L65出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajplung.00030.2018
关键词
atomic force microscopy; cytoskeleton; fibroblasts; idiopathic pulmonary fibrosis; alpha-smooth muscle actin
资金
- National Health and Medical Research Council, Australia Fellowship [1032695]
- University of Groningen
- European Union
- Rosalind Franklin Fellowship
- RESPIRE2 fellowship
- European Respiratory Society
- Longfonds Junior Investigators Grant [4.2.16.132JO]
Idiopathic pulmonary fibrosis (IPF) is a lethal lung disease involving degenerative breathing capacity. Fibrotic disease is driven by dysregulation in mechanical forces at the organ, tissue, and cellular level. While it is known that, in certain pathologies, diseased cells are stiffer than healthy cells, it is not known if fibroblasts derived from patients with IPF are stiffer than their normal counterparts. Using IPF patient-derived cell cultures, we measured the stiffness of individual lung fibroblasts via high-resolution force maps using atomic force microscopy. Fibroblasts from patients with IPF were stiffer and had an augmented cytoskeletal response to transforming growth factor-beta 1 compared with fibroblasts from donors without IPF. The results from this novel study indicate that the increased stiffness of lung fibroblasts of IPF patients may contribute to the increased rigidity of fibrotic lung tissue.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据