期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 17, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/ijms17081153
关键词
exon junction complex (EJC); Y14; pre-mRNA splicing; mitotic cell-cycle
资金
- Japan Society for the Promotion of Science (JSPS) [23510234, 26290062]
- Grants-in-Aid for Scientific Research [15K14451, 26290062, 23510234] Funding Source: KAKEN
The exon junction complex (EJC) that is deposited onto spliced mRNAs upstream of exon-exon junctions plays important roles in multiple post-splicing gene expression events, such as mRNA export, surveillance, localization, and translation. However, a direct role for the human EJC in pre-mRNA splicing has not been fully understood. Using HeLa cells, we depleted one of the EJC core components, Y14, and the resulting transcriptome was analyzed by deep sequencing (RNA-Seq) and confirmed by RT-PCR. We found that Y14 is required for efficient and faithful splicing of a group of transcripts that is enriched in short intron-containing genes involved in mitotic cell-cycle progression. Tethering of EJC core components (Y14, eIF4AIII or MAGOH) to a model reporter pre-mRNA harboring a short intron showed that these core components are prerequisites for the splicing activation. Taken together, we conclude that the EJC core assembled on pre-mRNA is critical for efficient and faithful splicing of a specific subset of short introns in mitotic cell cycle-related genes.
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