期刊
EXPERIMENTAL GERONTOLOGY
卷 65, 期 -, 页码 42-45出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.exger.2015.03.003
关键词
Lumbrical muscle; Contractility; Sarcopenia
资金
- NIH [R01-AR063649, P30-AG024824]
A progressive loss of skeletal muscle mass and force generating capacity occurs with aging. Mice are commonly used in the study of aging-associated changes in muscle size and strength, with most models of aging demonstrating 15-35% reductions in muscle mass, cross-sectional area (CSA), maximum isometric force production (P-o) and specific force (sP(o)), which is P-o/CSA. The lumbrical muscle of the mouse forepaw is exceptionally small, with corresponding short diffusion distances that make it ideal for in vitro pharmacological studies and measurements of contractile properties. However, the aging-associated changes in lumbrical function have not previously been reported. To address this, we tested the hypothesis that compared to adult (12 month old) mice, the forepaw lumbrical muscles of old (30 month old) mice exhibit aging-related declines in size and force production similar to those observed in larger limb muscles. We found that the forepaw lumbricals were composed exclusively of fibers with type II myosin heavy chain isoforms, and that the muscles accumulated connective tissue with aging. There were no differences in the number of fibers per whole-muscle cross-section or in muscle fiber CSA. The whole muscle CSA in old mice was increased by 17%, but the total CSA of all muscle fibers in a whole-muscle cross-section was not different. No difference in Po was observed, and while sPo normalized to total muscle CSA was decreased in old mice by 22%, normalizing Po by the total muscle fiber CSA resulted in no difference in sPo. Combined, these results indicate that forepaw lumbrical muscles from 30 month old mice are largely protected from the aging-associated declines in size and force production that are typically observed in larger limb muscles. (C) 2015 Elsevier Inc. All rights reserved.
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