期刊
Journal of Breath Research
卷 10, 期 3, 页码 -出版社
IOP PUBLISHING LTD
DOI: 10.1088/1752-7155/10/3/037102
关键词
selected ion flow tube mass spectrometry; volatile metabolites; cystic fibrosis; Pseudomonas aeruginosa; Burkholderia cepacia complex; Staphylococcus aureus; Stenotrophomonas maltophilia
资金
- Czech Science Foundation (GACR) [14-14534S]
As a contribution to the continuing search for breath biomarkers of lung and airways infection in patients with cystic fibrosis, CF, we have analysed the volatile metabolites released in vitro by Pseudomonas aeruginosa and other bacteria involved in respiratory infections in these patients, i.e. those belonging to the Burkholderia cepacia complex, Staphylococcus aureus or Stenotrophomonas maltophilia. These opportunistic pathogens are generally harmless to healthy people but they may cause serious infections in patients with severe underlying disease or impaired immunity such as CF patients. Volatile organic compounds emitted from the cultures of strains belonging to the above-mentioned four taxa were analysed by selected ion flow tube mass spectrometry. In order to minimize the effect of differences in media composition all strains were cultured in three different liquid media. Multivariate statistical analysis reveals that the four taxa can be well discriminated by the differences in the headspace VOC concentration profiles. The compounds that should be targeted in breath as potential biomarkers of airway infection were identified for each of these taxa of CF pathogens.
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