期刊
EXPERIMENTAL DERMATOLOGY
卷 24, 期 2, 页码 157-159出版社
WILEY-BLACKWELL
DOI: 10.1111/exd.12594
关键词
analysis; histochemistry; immunofluorescence; immunohistochemistry; method
类别
资金
- NIAMS [P30-AR057217]
- National Space Biomedical Research Institute (NSBRI) through NASA [NCC 9-58]
Colorimetric staining techniques such as immunohistochemistry (IHC), immunofluorescence (IF) and histochemistry (HC) provide useful information regarding the localization and relative amount of a molecule/substance in skin. We have developed a novel, straightforward method to assess colorimetric staining by combining features from two open-source software programs. As a proof of principle, we demonstrate the utility of this approach by analysing changes in skin melanin deposition during the radiation-induced tanning response of Yucatan mini-pigs. This method includes a visualization step to validate the accuracy of colour selection before quantitation to ensure accuracy. The data show that this method is robust and will provide a means to obtain accurate comparative analyses of staining in IHC/IF/HC samples.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据