Effects of purified zearalenone on selected immunological measurements of blood in post-weaning gilts

Zearalenone (ZEA), an estrogenic mycotoxin, is produced mainly by Fusarium fungi. Previous studies have indicated that acute ZEA exposure induced various damages in different species; however, its transparent hematotoxicity in female piglets at dietary levels of 1.1 to 3.2 mg/kg has not been shown. The present study was conducted to investigate the effects of dietary ZEA (1.1-3.2 mg/kg) on hematology, T lymphocyte subset, immunoglobulin, antibody titer, lymphocyte proliferation rate (LPR), and interleukin-2 (IL-2) in peripheral blood of post-weaning gilts. A total of 20 female piglets (Landrace x Yorkshire x Duroc), weaned at 42 d with an average body weight of 10.36 +/- 1.21 kg were used in the study. Female piglets were kept in a temperature controlled room, divided into four treatments, and fed a diet based on corn-soybean meal-fishmeal-whey, with an addition of 0, 1.1, 2.0, or 3.2 mg/kg purified ZEA for 18 d ad libitum. Feed intake and refusal were measured daily and individual pigs were weighed weekly. Blood and serum samples were collected for selected immunological measurements. Female piglets fed different levels of dietary ZEA grew similarly with no difference in feed intake. Hematological values including leukocytes, platelets, lymphocytes, hematocrit, and mean corpuscular hemoglobin (MCH) decreased linearly (P < 0.05) as dietary ZEA increased. Female piglets fed diets containing 2.0 mg/kg ZEA or greater showed significantly decreased CD4(+)CD8(+), CD4(+), and CD4(+)/CD8(+) in comparison to the control (P < 0.05), whereas CD8(+) was significantly increased (P = 0.026) in the gilts which were fed the diet containing 3.2 mg/kg ZEA. Serum immunoglobulin G (IgG) and the antibody titer on d 18 were reduced linearly as dietary ZEA levels increased (P < 0.001). Linear decrease in LPR was observed (P < 0.05). Female piglets fed diets containing 2.0 mg/kg ZEA or more showed significantly decreased IL-2 in comparison to the control (P < 0.05). The results suggested that dietary ZEA at the levels of 1.1 to 3.2 mg/kg can induce different degrees of hematotoxicity and negatively affect immune function in female piglets. (C) 2016, Chinese Association of Animal Science and Veterinary Medicine. Production and hosting by Elsevier B. V. on behalf of KeAi Communications Co., Ltd.