期刊
EXPERIMENTAL CELL RESEARCH
卷 336, 期 1, 页码 94-99出版社
ELSEVIER INC
DOI: 10.1016/j.yexcr.2015.05.009
关键词
Satellite glial cells; Trigeminal ganglion; Oxaliplatin; Carbenoxolone; Connexin 43; Chemotherapy-induced pain
资金
- Oticon fonden
- Knud Hojgaards Fond
- Israel Science Foundation [508/13]
- US-Israel Binational Science Foundation [2011044]
- Div Of Biological Infrastructure
- Direct For Biological Sciences [2011044] Funding Source: National Science Foundation
Communications between satellite glial cells and neighboring neurons within sensory ganglia may contribute to neuropathic and inflammatory pain. To elucidate the role of satellite glial cells in chemotherapy-induced pain, we examined the effects of oxaliplatin on the gap junction-mediated coupling between these cells. We also examined whether the gap junction blocker, carbenoxolone, can reverse the coupling. Primary cultures of mice trigeminal ganglia, 24-48 h after cell isolation, were used. Satellite glial cells were injected with Lucifer yellow in the presence or absence of oxaliplatin (60 mu M). In addition, the effect of carbenoxolone (100 mu M) on coupling, and the expression of connexin 43 proteins were evaluated. Dye coupling between adjacent satellite glial cells was significantly increased (2.3-fold, P<0.05) following a 2 h incubation with oxaliplatin. Adding carbenoxolone to the oxaliplatin-treated cultures reversed oxaliplatin-evoked coupling to baseline (P<0.05). Immunostaining showed no difference between expression of connexin 43 in control and oxaliplatin-treated cultures. Our findings indicated that oxaliplatin-increased gap junction-mediated coupling between satellite glial cells in primary cultures of mouse trigeminal ganglia, and carbenoxolone reversed this effect. Hence, it is proposed that increased gap junction-mediated coupling was seen between satellite glial cells in TG. This observation together with our previous data obtained from a behavioral study suggests that this phenomenon might contribute to chemotherapy-induced nociception following oxaliplatin treatment. (C) 2015 Elsevier Inc. All rights reserved.
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