期刊
ADVANCED MATERIALS
卷 30, 期 13, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adma.201705799
关键词
3D N IR-II molecular imaging; high-performance NIR-II bioconjugate; live ovary imaging; multicolor live molecular imaging; NIR-II confocal
类别
资金
- National Institutes of Health NIH [DP1-NS-105737]
- Office of Science (BER)
- NCI Cancer Center Nanotechnology Excellence Grant (CCNE-TR)
- Calbrain Program
- National Institutes of Health [R01 HL127113-01A1]
- Shenzhen Key Lab Funding Grant [ZDSYS201505291525382]
- Shenzhen Peacock Program Grant [KQTD20140630160825828]
Greatly reduced scattering in the second near-infrared (N I R - 11) region (1000 1700 nm) opens up many new exciting avenues of bioimaging research, yet N I R-11 fluorescence imaging is mostly implemented by using nontargeted fluorophores or wide-field imaging setups, limiting the signalto-background ratio and imaging penetration depth due to poor specific binding and out-of-focus signals. A newly developed high-performance NIR-II bioconjugate enables targeted imaging of a specific organ in the living body with high quality. Combined with a home-built NIR-II confocal set-up, the enhanced imaging technique allows 900 gm-deep 3D organ imaging without tissue clearing techniques. Bioconjugation of two hormones to nonoverlapping NIR-II fluorophores facilitates two-color imaging of different receptors, demonstrating unprecedented multicolor live molecular imaging across the NIR-II window. This deep tissue imaging of specific receptors in live animals allows development of noninvasive molecular imaging of multifarious models of normal and neoplastic organs in vivo, beyond the traditional visible to NIR-I range. The developed NIR-II fluorescence microscopy will become a powerful imaging technique for deep tissue imaging without any physical sectioning or clearing treatment of the tissue.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据