期刊
ADVANCED MATERIALS
卷 30, 期 27, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adma.201800242
关键词
bioprinting; digital light prototyping; digital micromirror devices; microfluidics; multimaterials
类别
资金
- Office of Naval Research Young National Investigator Award
- National Institutes of Health [AR057837, DE021468, D005865, AR068258, AR066193, EB022403, EB021148, EB021857]
- Presidential Early Career Award for Scientists and Engineers (PECASE)
- National Cancer Institute of the National Institutes of Health Pathway to Independence Award [K99CA201603]
- Fonds de recherche du Quebec - Sante (FRQS) postdoctoral fellowship
- (I2C plan) Xunta de Galicia
- Air Force Office of Sponsored Research [FA9550-15-1-0273, 2017N000114]
- Brigham and Women's Hospital President Betsy Nabel, MD
- Reny family
- NATIONAL CANCER INSTITUTE [K99CA201603] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES [R21AR068258, R01AR057837, R01AR066193] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [R21EB021148, R21EB022403, R01EB021857] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH [R01DE021468] Funding Source: NIH RePORTER
A stereolithography-based bioprinting platform for multimaterial fabrication of heterogeneous hydrogel constructs is presented. Dynamic patterning by a digital micromirror device, synchronized by a moving stage and a microfluidic device containing four on/off pneumatic valves, is used to create 3D constructs. The novel microfluidic device is capable of fast switching between different (cell-loaded) hydrogel bioinks, to achieve layer-by-layer multimaterial bioprinting. Compared to conventional stereolithography-based bioprinters, the system provides the unique advantage of multimaterial fabrication capability at high spatial resolution. To demonstrate the multimaterial capacity of this system, a variety of hydrogel constructs are generated, including those based on poly(ethylene glycol) diacrylate (PEGDA) and gelatin methacryloyl (GelMA). The biocompatibility of this system is validated by introducing cell-laden GelMA into the microfluidic device and fabricating cellularized constructs. A pattern of a PEGDA frame and three different concentrations of GelMA, loaded with vascular endothelial growth factor, are further assessed for its neovascularization potential in a rat model. The proposed system provides a robust platform for bioprinting of high-fidelity multimaterial microstructures on demand for applications in tissue engineering, regenerative medicine, and biosensing, which are otherwise not readily achievable at high speed with conventional stereolithographic biofabrication platforms.
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