期刊
ACTA HISTOCHEMICA ET CYTOCHEMICA
卷 51, 期 2, 页码 93-100出版社
JAPAN SOC HISTOCHEMISTRY & CYTOCHEMISTRY
DOI: 10.1267/ahc.17039
关键词
DNA methylation; folliculogenesis; HELMET; apoptosis; epigenetics
类别
资金
- National Natural Science Foundation of China [81202175]
- Natural Science Foundation of Fujian Province [2012J05149]
- outstanding youth project in colleges and universities of Fujian Province [JA13132]
- Japan Society for the Promotion of Science [18390060, 16K15173]
- Grants-in-Aid for Scientific Research [16K15173, 16H05813] Funding Source: KAKEN
For a better understanding of epigenetic regulation of cell differentiation, it is important to analyze DNA methylation at a specific site. In this study, we examined changes in the methylation level of CCGG and GATCG sites during mouse folliculogenesis in paraffin-embedded sections of mouse ovaries. For the purpose, we used a new method, histo endonuclease-linked detection of methylation sites of DNA (HELMET), designed to detect methylation sites of DNA with a specific sequence in a tissue section. Unlike the global level of DNA methylation, which was no change in immunohistochemical staining of 5-methylcytosine throughout folliculogenesis, we found that there were hypermethylation of CCGG and GATCG sites in most of the granulosa cells of tertiary follicles compared to that of primary and secondary follicles. Interestingly, TUNEL-positive granulosa cells, which were frequent in mammalian folliculogenesis, became markedly Hpa II-reactive and Sau3A I-reactive, indicating that the CCGG and GATCG sites may be preferentially demethylated during apoptosis.
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