期刊
ACTA BIOMATERIALIA
卷 71, 期 -, 页码 496-509出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2018.03.019
关键词
Adipose-derived stem cells; Nucleus pulposus; Hydrogel-like cells delivery system; Genipin; Tissue engineering
资金
- National Natural Science Foundation of China [81401822, 81472114, 81401011, 81572177]
- General Science and Technology Planning Project [2016C33151]
- Zhejiang Provincial Natural Science Foundation of China [Q14H060007, Y13H060001]
- General Research Program of Zhejiang Provincial Department of Health [2016142263, 2016146428]
Nucleus pulposus (NP) degeneration is usually the origin of intervertebral disc degeneration and consequent lower back pain. Although adipose-derived stem cell (ADSC)-based therapy is regarded to be promising for the treatment of degenerated NP, there is a lack of viable cell carriers to transplant ADSCs into the NP while maintaining cell function. In this study, we developed a type II collagen/chondroitin sulfate (CS) composite hydrogel-like ADSC (CCSA) delivery system with genipin as the cross linking agent. The induction effect of the scaffold on ADSC differentiation was studied in vitro, and a rat coccygeal vertebrae degeneration model was used to investigate the regenerative effect of the CCSA system on the degenerated NP in vivo. The results showed that the CCSA delivery system cross linked with 0.02% genipin was biocompatible and promoted the expressions of NP-specific genes. After the injection of the CCSA system, the disc height, water content, extracellular matrix synthesis, and structure of the degenerated NP were partly restored. Our CCSA delivery system uses minimally invasive approaches to promote the regeneration of degenerated NP and provides an exciting new avenue for the treatment of degenerative disc disease.
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