期刊
ACTA BIOMATERIALIA
卷 72, 期 -, 页码 137-149出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2018.03.027
关键词
Electrospun fibers; Elastin-like recombinamers; Click reaction; Wound healing
资金
- European Commission (ELASTISLET) [646075]
- European Commission (BIOGEL) [642687]
- Ministerio de Economia y Competitividad of the Spanish Government [MAT2015-68901-R, MAT2016-78903-R, PCIN-2015-010]
- Junta de Castilla y Leon [VA015U16]
- Marie Curie Actions (MSCA) [642687] Funding Source: Marie Curie Actions (MSCA)
Herein we present a system to obtain fibers from clickable elastin-like recombinamers (ELRs) that cross-link in situ during the electrospinning process itself, with no need for any further treatment to stabilize them. These ELR-click fibers are completely stable under in vitro conditions. A wrinkled fiber morphology is obtained. In addition to a random fiber orientation, oriented fibers with a high degree of alignment and coherence can also be obtained by using a rotational electrode. The production of multicomponent fibers means that different functionalities, such as cell-adhesion domains (RGD peptides), can be incorporated into them. In a subsequent study, two main cell lines present in the dermis and epidermis, namely keratinocytes and fibroblasts, were cultured on top of the ELR-click fibers. Adhesion, proliferation, fluorescence, immunostaining and histology studies showed the cytocompatibility of these scaffolds, thus suggesting their possible use for wound dressings in skin tissue engineering applications. Statement of significance For the first time stable electrospun bioactive fibers are obtained by the in situ mixing of two clickable ELR components previously described by Gonzalez et al (Acta Biomaterialia 2014). This work describes an efficient system to prepare fibrous scaffolds based on peptidic polymers by electrospinning without the need of crosslinking agents that could be harmful for cells or living tissues. These bioactive fibers support cell growth due to the inclusion of RGD motifs (Staubli et al. Biomaterials 2017). Finally, the in vitro biocompatibility of the two main cell types found in the outer layers of skin, fibroblasts and keratinocytes, indicates that this system is of great interest to prepare elastic artificial skin substitutes for wound healing applications. (C) 2018 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据