期刊
ACTA BIOMATERIALIA
卷 73, 期 -, 页码 127-140出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2018.04.012
关键词
Meniscus; Scaffold; Electrospinning; PCL; Decellularize; Tissue engineering
资金
- National Natural Science Foundation of China [81472092]
- High Technology Research and Development Program of China [2012AA020502, 2015AA020303]
- National Key Research and Development Program of China [2017YFC1104102, 2017YFC1103404]
- Natural Science Foundation of Beijing Municipality [7172203]
- Beijing Science and Technology Development Foundation [Z161100005016059]
Low vascularization in meniscus limits its regeneration ability after injury, and tissue engineering is the most promising method to achieve meniscus regeneration. In this study, we fabricated a kind of composite scaffold by decellularized meniscus extracellular matrix/polycaprolactone (DMECM/PCL) electrospinning fibers and porous DMECM, in which DMECM/PCL fibers were used as reinforcing component. The tensile modulus of the composite scaffold in longitudinal and crosswise directions were 8.5 +/- 1.9 and 2.3 +/- 0.3 MPa, respectively. Besides that, the DMECM/PCL electrospinning fibers enhanced suture resistance of the composite scaffold more than 5 times than DMECM scaffold effectively. In vitro cytocompatibility showed that the porous structure provided by DMECM component facilitated meniscus cells' proliferation. DMECM was also the main component to regulate cell behaviors, which promoted meniscus cells expressing extracellular matrix related genes such as COL I, COL II, SOX9 and AGG. Rabbits with total meniscectomy were used as animal model to evaluated the composited scaffolds performance in vivo at 3 and 6 months. Results showed that rabbits with scaffold implanting could regenerate neo-menisci in both time points. The neo-menisci had similar histology structure and biochemical content with native menisci. Although neo-menisci had inferior tensile modulus than native ones, its modulus was improved with implanting time prolonging. MRI imaging showed the signal of neo-meniscus in the body is clear, and X-ray imaging of knee joints demonstrated the implantation of scaffolds could relief joint space narrowing. Moreover, rabbits with neo-menisci had better cartilage condition in femoral condyle and tibial plateau compared than meniscectomy group. (C) 2018 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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