期刊
ACS NANO
卷 12, 期 4, 页码 3378-3384出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.7b08965
关键词
sepsis; organ failure; IL-3; point-of-care; electrochemical sensing
类别
资金
- U.S. Department NIH grants [R21-CA205322, R01-HL113156, K99-CA201248, R01-CA204019, R01-EB010011, R01-EB00462605A1, P01-CA069246]
- Liz Tilberis Award Fund
- MGH scholar fund
- Andrew L. Warshaw, M.D. Institute for Pancreatic Cancer Research
- Lustgarten Foundation
- National Research Foundation of Korea [NRF-2017M3A9B4025699, NRF-2017M3A9B4025709]
- NATIONAL CANCER INSTITUTE [R01CA204019, K99CA201248, T32CA079443, R21CA205322, R00CA201248, P01CA069246] Funding Source: NIH RePORTER
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL113156] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [R01EB004626, R01EB010011] Funding Source: NIH RePORTER
Sepsis is an often fatal condition that arises when the immune response to an infection causes widespread systemic organ injury. A critical unmet need in combating sepsis is the lack of accurate early biomarkers that produce actionable results in busy clinical settings. Here, we report the development of a point-of-care platform for rapid sepsis detection. Termed IBS (integrated biosensor for sepsis), our approach leverages (i) the pathophysiological role of cytokine interleukin-3 (IL-3) in early sepsis and (ii) a hybrid magneto-electrochemical sensor for IL-3 detection. The developed platform produces test results within 1 h from native blood samples and detects IL-3 at a sensitivity of <10 pg/mL; this performance is >5-times faster and >10-times more sensitive than conventional enzyme-linked immunoadsorbent assays, the current gold standard. Using clinical samples, we show that elevated plasma IL-3 levels are associated with high organ failure rate and thus greater risk of mortality, confirming the potential of IL-3 as a sepsis diagnostic biomarker. With further system development (e.g., full automation, data security measures) and rigorous validation studies, the compact and fast IBS could be a practical clinical tool for timely diagnosis and proactive treatment of sepsis.
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