期刊
ACS NANO
卷 12, 期 8, 页码 8540-8546出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.8b04080
关键词
Brownian; scanning FCS; free and trapped diffusion; actin cytoskeleton; hindered diffusion dynamics; diffusion modes
类别
资金
- Wellcome Trust [091911]
- Wolfson Foundation
- Medical Research Council (MRC) [MC UU 12010, G0902418, MC UU 12025]
- MRC/BBSRC/EPSRC [MR/K01577X/1]
- University of Oxford
- BBSRC [BB/P026354/1] Funding Source: UKRI
- MRC [MC_UU_12010/9, MR/K01577X/1, MC_UU_00008/9, G0902418] Funding Source: UKRI
Cells rely on versatile diffusion dynamics in their plasma membrane. Quantification of this often heterogeneous diffusion is essential to the understanding of cell regulation and function. Yet such measurements remain a major challenge in cell biology, usually due to low sampling throughput, a necessity for dedicated equipment, sophisticated fluorescent label strategies, and limited sensitivity. Here, we introduce a robust, broadly applicable statistical analysis pipeline for large scanning fluorescence correlation spectroscopy data sets, which uncovers the nanoscale heterogeneity of the plasma membrane in living cells by differentiating free from hindered diffusion modes of fluorescent lipid and protein analogues.
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