期刊
ACS NANO
卷 12, 期 5, 页码 4156-4163出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.8b00025
关键词
integrated 3D nanoscopy; gold nanorod; anisotropy; superlocalization; polarization
类别
资金
- Basic Science Research Program of the National Research Foundation of Korea (NRF) - Ministry of Education, Science and Technology [2015R1A2A2A01003839]
- National Science Foundation [CBET-1604612]
- Directorate For Engineering [1604612] Funding Source: National Science Foundation
Three-dimensional (3D) orientations of individual anisotropic plasmonic nanoparticles in aggregates were observed in real time by integrated light sheet super-resolution microscopy (iLSRM). Asymmetric light scattering of a gold nanorod (AuNR) was used to trigger signals based on the polarizer angle. Controlled photoswitching was achieved by turning the polarizer and obtaining a series of images at different polarization directions. 3D subdiffraction-limited super-resolution images were obtained by superlocalization of scattering signals as a function of the anisotropic optical properties of AuNRs. Varying the polarizer angle allowed resolution of the orientation of individual AuNRs. 3D images of individual nano particles were resolved in aggregated regions, resulting in as low as 64 nm axial resolution and 28 nm spatial resolution. The proposed imaging setup and localization approach demonstrates a convenient method for imaging under a noisy environment where the majority of scattering noise comes from cellular components. This integrated 3D iLSRM and localization technique was shown to be reliable and useful in the field of 3D nonfluorescence super-resolution imaging.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据